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Point 1

Human dermis fibroblasts proliferation. Acoustic stimulation conditions: 110 Hz, Intensity 74, 30 min. Media: 15% FBS in DMEM

→ Acoustic vibration stimulation is given to cells in 15% FBS culture once a day for 3 days. As a result of measuring the number of cells by the ADAM automatic cell counting method, observation of cell proliferation stimulated on Day 3

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Point 2

Col3a1 gene expression confirmation. Mode 100Hz 68 contrast. B mode 110Hz 74 contrast.

→ COL3A1 expression was significantly increased. (Gene expressed when collagen, a major protein constituting skin, blood vessels, and cartilage, is produced.)

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Point 3

Less cytokines.

Acoustic stimulation conditions: B Mode 110 Hz, Intensity 74

→IL-1β expression induced in human dermal fibroblasts through inflammatory metabolite arachidonic acid is reduced by acoustic vibration conditions. (A decrease in cytokine IL-1β means a decrease in inflammation.)